active gαi-gtp [26901]
[30 µl]
catalog number: 26901
gene symbol: gnai
description: anti-active gα i mouse monoclonal antibody
background: heterotrimeric g proteins are critical cellular signal transducers. gα irepresents one sub-family of g proteins that could mediate the inhibition of adenylyl cyclases. other biochemical and physiological functions of gα i proteins are being explored.
immunogen: recombinant full length protein of active gα i1
applications: ip, ihc
recommended dilutions: 1 µg for 1~2 mg total cellular proteins for ip;
1:100 dilution for ihc
concentration: 1 mg/ml
host: mouse
format: liquid
clonality: monoclonal
isotype: igg1
purity: purified from ascites
storage buffer:
preservative: no
constituents: pbs (without mg 2+ and ca 2+ ), ph 7.4, 150 mm nacl, 50% glycerol
species reactivity: anti-active gα i antibody recognizes active gα i1, gα i2, and gα i3 of vertebrates.
storage conditions: store at -20°c. avoid freeze / thaw cycles
immunoprecipitation/western blot:
gαi activation assay.
a. cho cells were transfected with wild-type gα i1 (lanes 1 and 2) or constitutively active gα i1-q204l (lane 3). cell lysates were treated with gdp(lane 1) or gtpγs (lane 3). lysates were then incubated with an anti-active gα i monoclonal antibody (cat. no.26901) (top panel). the precipitated active gα iwas immunoblotted with an anti-gα i monoclonal antibody (cat. no. 26003). the bottom panel shows the western blot with anti-gα i monoclonal antibody (cat.no. 26003) of the cell lysates.
b. hek293 cells stably expressing human m2 machr were treated with (lane 2) or without (lane 1)carbachol. cell lysates were then incubated with an anti-active gα i monoclonal antibody (cat. no. 26901) (top panel).the precipitated active gα i was immunoblotted with an anti-gα i rabbit polyclonal antibody (cat. no. 21006). the bottom panel shows the western blot with anti-tubulin of the cell lysates.
publications:
1.
biochem j. 2013 feb 15;450(1):209-19
2.
j clin endocrinol metab. 2012 oct;97(10):e1851-61
3.
cancer res. 2012 jan 1;72(1):122-32
4.
endocrinology. 2012 oct;153(10):4918-28
5.
cell signal. 2011 mar;23(3):550-4
6.
biochim biophys acta. 2011 mar;1813(3):466-72
7.
steroids volume 77, issue 5, april 2012, pages 424–432
8.
j biol chem. 2010 aug 27;285(35):26976-86
9.
proc natl acad sci u s a. 2010 jan 12;107(2):628-32
10.
mol biol cell. 2014 nov 5;25(22):3654-71
11.
mol cell biol. 2014 nov 15;34(22):4186-99
12.
j biol chem. 2014 may 2;289(18):12748-58
武汉纽斯特生物技术有限公司
18972339958
中国 武汉